Xylella fastidiosa subsp. sandyi CFBP8356 Genome sequencing and assembly

Xylella fastidiosa is a bacterial plant pathogen disseminated across the Americas, while its presence is currently described in several European countries. Seven strains, available at the CIRM-CFBP (http://www.angers-nantes.inra.fr/cfbp/) were selected. Six strains were isolated in the USA from Vitis rotundifolia (CFBP7969), Vitis vinifera (CFBP 7970), Vitis SP (CFBP8351), Prunus dulcis (CFBP 8071) and Ambrosia artemifolia (CFBP 8082) and belong to X. fastidiosa subsp. fastidiosa. One strain was isolated in the USA from Vinca sp. (CFBP 8078) and belongs to X. fastidiosa subsp. multiplex. One strain was isolated from a coffee plant imported from the Costa Rica and intercepted in France (CFBP 8356) and belongs to X. fastidiosa subsp. sandyi. The taxonomic assignation to a subpecies was based on multiprimer PCR (Hernandez-Martinez et al., 2006), MLST scheme using 7 housekeeping genes (Yuan et al., 2010) and whole-genome sequencing unsing the Illumina Mi-Seq technology.

苛养木杆菌（Xylella fastidiosa）是一种广泛分布于美洲的植物病原细菌，目前已有多个欧洲国家报道其存在。本研究选取了保藏于法国农业科学研究院植物病原细菌保藏库（CIRM-CFBP，http://www.angers-nantes.inra.fr/cfbp/）的7个菌株。其中6个菌株分离自美国，宿主分别为圆叶葡萄（Vitis rotundifolia，CFBP7969）、欧洲葡萄（Vitis vinifera，CFBP 7970）、葡萄属未知种（Vitis SP，CFBP8351）、扁桃（Prunus dulcis，CFBP 8071）以及豚草（Ambrosia artemifolia，CFBP 8082），上述菌株均隶属于苛养木杆菌指名亚种（X. fastidiosa subsp. fastidiosa）。另有1个菌株分离自美国的长春花属植物（Vinca sp.，CFBP 8078），隶属于苛养木杆菌多型亚种（X. fastidiosa subsp. multiplex）。还有1个菌株分离自从哥斯达黎加进口并在法国截获的咖啡植株（CFBP 8356），隶属于苛养木杆菌桑迪亚种（X. fastidiosa subsp. sandyi）。所有菌株的亚种分类鉴定均基于多引物PCR技术（Hernandez-Martinez等，2006）、包含7个持家基因的多位点序列分型（Multi-Locus Sequence Typing，MLST）方案（Yuan等，2010），以及采用Illumina MiSeq技术完成的全基因组测序。