Utility and limitation of universal PCR screening for SARS-CoV-2 on hospital admission

Three files include the statistical data and graph made by graph pad prism.<br>Figure 1 describes the correlation of two PCR targets in COBAS SARS-CoV-2 PCR. The Ct values of target 1 (ORF1ab gene) and target 2 (E gene) from 111 PCR tests are plotted on the graph. Ct value of target 1 and target 2 were plotted on the graph to show the correlation of two targets. Negative PCR result was shown as 40. Red solid line shows the linear regression of 111 PCR results with the equation of regression line and R-square value.6 PCR tests showed negative result in target 1, but showed positive result in target 2. 14 PCR tests showed positive result in target 1, but showed negative result in target 2.<br>Figure 2 describes the transition of the number of PCR screening tests and positive results during the study period.The black line (a) shows the number of monthly PCR screening tests upon admission to our department. The red line shows the number of positive PCR results. For comparing our PCR positive results and local COVID-19 spread, the monthly COVID-19 cases from public data base was shown in (b). The monthly patients number in Fukuoka prefecture (approximately 5 million population) was calculated by the daily cases available at “https://www.mhlw.go.jp/stf/covid-19/open-data.html”<br>Figure 3 describes the PCR positivity after COVID-19 onset.All Ct value of target 1 from 66 cases with known COVID-19 onset date were grouped according to the date after COVID-19 onset and plotted on the graph (a). Negative PCR result was shown as Ct value of 40. Each red dot shows the individual test results with the mean and 95% CI in the groups. Based on the tests results shown in (a), PCR positivity was calculated in (b). There was no PCR test conducted at 91 - 100 days after COVID-19 onset.

本数据集包含3份由GraphPad Prism生成的统计数据与可视化图表。
图1展示了COBAS严重急性呼吸综合征冠状病毒2（SARS-CoV-2）聚合酶链式反应（PCR）检测中两个靶标间的相关性。本图表绘制了111次PCR检测中靶标1（ORF1ab基因）与靶标2（E基因）的循环阈值（Cycle Threshold，Ct）值。以两个靶标的Ct值为坐标轴绘图，以呈现二者的相关性。PCR检测阴性结果以Ct值40表示。红色实线为111次PCR检测结果的线性回归拟合线，并标注了回归方程与决定系数（R²）。另有6次PCR检测结果为靶标1阴性、靶标2阳性，14次为靶标1阳性、靶标2阴性。

图2展示了研究周期内PCR筛查检测量与阳性结果数的变化趋势。黑线（a）代表本院收治患者的月度入院PCR筛查检测量，红线代表月度PCR阳性检测结果数。为对比本院PCR阳性结果与当地新冠疫情传播情况，图表（b）展示了来自公共数据库的月度新冠确诊病例数。福冈县（人口约500万）的月度病例数通过日本厚生劳动省官方公开数据平台（https://www.mhlw.go.jp/stf/covid-19/open-data.html）提供的每日新增病例计算得出。

图3展示了新冠病毒感染发病后的PCR检测阳性率变化。针对66例已知发病日期的病例，根据其感染发病后的天数分组，绘制了靶标1的Ct值分布（图a）。PCR阴性结果以Ct值40表示。每个红色圆点代表单份检测结果，分组内同时标注了均值与95%置信区间（Confidence Interval，CI）。基于图a的检测结果，在图b中计算并展示了PCR检测阳性率。在感染发病后91至100天期间，未开展相关PCR检测。