Transgenerational effect of Chlordecone [ChIP-seq]

The environmental factors such as pesticides could affect the developmental processes and cause the phenotypic changes. Moreover, the changes could be transferred to a subsequent generation via gametes. In this project we studied whether the pesticide chlordecone could cause the developmental effects and cause the transgenerational inheritance in outbred mouse strain. Mice were treated during development at dose 100 µg/kg/day and the progeny of exposed mice were crossed for three generations. The testes of the third generations males were used for anti-H3K4me3 ChiP-seq analysis. We used two biological replicates for each condition. H3K4me3 ChIP-seq on testes.

诸如农药类的环境因素可影响生物体发育进程并引发表型改变，且此类改变可通过配子传递至子代。本研究旨在探究农药十氯酮（chlordecone）是否可在远交系小鼠品系中诱导发育效应及跨代遗传现象。实验中，小鼠于发育阶段以100 µg/kg/天的剂量接受处理，暴露小鼠的后代经连续三代交配繁育。取第三代雄性小鼠的睾丸开展抗H3K4me3染色质免疫沉淀测序（ChIP-seq）分析，每个实验条件均设置两个生物学重复。本实验针对小鼠睾丸进行H3K4me3 ChIP-seq检测。