scRNAseq of PC9 parental and persister cells

We performed scRNAseq of PC9 parental (PT) and persister cells (PS) at d1, d3 and d7 after being generated by treatment with BH3-mimetics. Trajectory analysis and differential expression along the pseudotime revealed the upregulation of the integrated stress response (ISR) and EMT signatures at early pseudotime, while gene expression profiles for cell cycle progression were upregulated at late pseudotime, characteristic for late PS and PT. scRNAseq of PC9 parental and persister cells at d1, d3 and d7 generated by treatment with BH3-mimetics

本研究对PC9亲本细胞（PT）与经BH3模拟物处理构建的持留细胞（PS），分别于处理后第1、3、7天开展单细胞RNA测序（single-cell RNA sequencing，scRNAseq）。沿伪时间轴进行轨迹分析与差异表达分析后发现，伪时间早期可检测到整合应激反应（ISR）与上皮间质转化（EMT）特征基因的上调表达；而细胞周期进程相关基因表达谱则在伪时间后期呈现上调趋势，该特征为晚期持留细胞和亲本细胞所特有。本研究的测序样本还包括经BH3模拟物处理构建的PC9亲本细胞与持留细胞，采样时间点同样为处理后第1、3、7天。