Analysis of transgenerational effects on DNA copy number aberrations in male mice exposed to continuous 20mGy/day gamma-rays for 400 days (Secondary screening for 0GyI family)

Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. C57BL/6JNrs male mice were maintained in non-irradiated room for 400 days from 8 weeks of age. This study investigated the De novo mutation by comparing the parents and children. This is secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan. Two-condition experiment, C57 BL/6JNrs tail tissue non-irradiated male (0GyIM), female (0GyIF) and their progenies (0GyI1 to 0GyI6) vs. reference male mouse.

持续低剂量率（low dose-rate, LDR）γ射线照射的跨代效应尚未得到充分研究。本研究选取8周龄起即在非照射环境中饲养400天的C57BL/6JNrs雄性小鼠为实验对象，通过比对亲代与子代个体，对新生突变（de novo mutation）展开研究。本次实验属于二次筛选，受日本青森县政府委托开展。本实验为双条件对照实验：以尾部组织取样的未接受照射的C57 BL/6JNrs雄性小鼠（0GyIM组）、雌性小鼠（0GyIF组）及其子代（0GyI1至0GyI6组）与参考雄性小鼠进行对照分析。