Gene expression in a Highly Tumorigenic Cells (HTC) purified from Urothelial Cancer. Homo sapiens

We used DNA microarrays to compare RNA transcript expression from distinct cell populations sorted by FACS with an antibody against the 67Kd laminin receptor (67LR), and showing distinct tumorigenicity in vivo. Overall design: Sorted cells were derived by digesting xenografted sw 780 tumors into single cell suspensions. Differential gene expression was assessed by direct comparisons of labeled moieties, using a dye-swap design and biological replication. Hybridizations were performed on Agilent (Santa Clara, CA) Whole Human Genome DNA microarrays (hgug4112a). Three different samples were analyzed: 1) Urothelial cancer cells expressing 67LR (67LR+, BRIGHT); 2) Urothelial cancer cells not expressing 67LR (67LR-, DIM); 3) Control sorted urothelial cancer cells (BULK, containing both BRIGHT and DIM cells). In this series we provide normalized single channel intensities, aligned with the corresponding raw expression data.

本研究采用DNA微阵列（DNA microarray）技术，对比经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）结合抗67Kd层粘连蛋白受体（67Kd laminin receptor, 67LR）抗体分选得到的不同细胞群的RNA转录本表达水平，上述细胞群在体内表现出显著差异的成瘤能力。

实验整体设计：分选所得细胞源自将异种移植的sw 780肿瘤消化制备的单细胞悬液。差异基因表达分析通过直接比较标记分子完成，实验采用染料交换设计与生物学重复。杂交实验在安捷伦（Agilent，美国加利福尼亚州圣克拉拉）全人类基因组DNA微阵列（hgug4112a）上开展。

本研究共分析3组样品：1）表达67LR的尿路上皮癌细胞（67LR+，BRIGHT）；2）不表达67LR的尿路上皮癌细胞（67LR-，DIM）；3）对照分选的尿路上皮癌细胞（BULK，包含BRIGHT与DIM两类细胞群）。本数据集提供经归一化处理的单通道信号强度值，并附带与之匹配的原始表达数据。