Somatic_mutations_in_normal_murine_tissue

Cancer is driven by mutation. Using Agilent exome hybridisation capture and Illumina massively parallel sequencing technology, we aim to sequence microRNAs plus the protein coding genome of a number of samples. Bespoke algorithms are being developed to identify the somatically acquired point mutations, insertions and deletions in these samples. This project will give unprecedented insights into mutational processes, cellular repair pathways and gene networks associated with cancer development.

癌症的发生由突变驱动。本研究采用安捷伦（Agilent）外显子组杂交捕获（exome hybridisation capture）技术与因美纳（Illumina）大规模并行测序（massively parallel sequencing）平台，拟对一批样本的微小RNA（microRNAs）及蛋白质编码基因组开展测序工作。研究团队正开发定制化算法，以鉴定这批样本中的体细胞获得性点突变（somatically acquired point mutations）、插入突变与缺失突变。本项目将为解析与癌症发生相关的突变过程、细胞修复通路及基因网络提供前所未有的研究视角。