Zea mays Transcriptome or Gene expression. Zea mays

At 35 DAP whole kernels (pericarp + endosperm + embryo) without glumes of green house grown ears of heterozygous (+/bt2-H2328), self-pollinated plants were visually divided into pools of phenotypically normal looking kernels (small indentation, slightly smaller than mutant kernels, genotype +/+ or +/bt2-H2328) and pools of phenotypically mutant kernels (plump, round kernels, slightly larger than normal kernels, genotype bt2-H2328/bt2-H2328). Pools consisted of 4 kernels. 3 different ears were used for a biological duplicate. Keywords: gene knock out Overall design: 4 arrays - maize

授粉后35天（Days After Pollination, DAP）时，采集杂合子（+/bt2-H2328）自交植株的温室种植果穗，分离得到去除颖壳（glumes）的完整籽粒，即果皮（pericarp）+胚乳（endosperm）+胚（embryo）。通过肉眼表型观察，将籽粒分为两组混样：表型正常籽粒组（籽粒具轻微凹陷，略小于突变体籽粒，基因型为+/+或+/bt2-H2328）与表型突变籽粒组（籽粒饱满圆润，略大于正常籽粒，基因型为bt2-H2328/bt2-H2328）。每组混样包含4个籽粒，共使用3个不同果穗完成生物学重复。关键词：基因敲除（gene knock out）。实验整体设计：共设置4张芯片，实验材料为玉米（maize）。