Chromosome-level genome assembly ofa hemiparasitic plant, Scurrula parasitica (Loranthaceae)

Scurrula parasitica(Loranthaceae) is a widespread aerial hemiparasitic plant in southwest China, recognized for its ecological roles and wide host range. As a representative of mistletoes in Santalales, it serves as a model for studying the genomic basis of aerial hemiparasitism. Here, we report a high-quality chromosome-level genome assembly of S. parasiticausing PacBio high-fidelity (HiFi)and Hi-C sequencing. The assembled genome spans 547.41 Mb with a contigN50 of 8.32Mb, and 97.54% of the sequence anchored to nine pseudochromosomes. Repetitive elements comprise 64.53% of the genome. We predicted 21,837 protein-coding genes, 96.05% of which were functionally annotated. Notably, 1,271 transcription factor genes were identified using PlantTFDB. Additionally, we annotated 8,407 non-coding RNAs, including 3,821 tRNAs, 3,076 snRNAs, 1,447 rRNAs, and 63 miRNAs. Genome quality was validated through multiple approaches, including BUSCO analysis, LTR assembly index, Illumina read mapping, and telomeric repeat detection, all of which support the high completeness and accuracy of the assembly. This chromosome-scale assembly provides a foundational resource for investigating gene family evolution, parasitic adaptation, and genome architecture in S. parasitica. The genome and associated datasets are publicly available, enabling future comparative and functional genomic studies in parasitic angiosperms.广寄生（Scurrula parasitica，桑寄生科Loranthaceae）是中国西南地区广泛分布的地上半寄生植物，以其多样的生态功能与宽泛的寄主范围为学界所熟知。作为檀香目Santalales槲寄生类群的代表性物种，其是研究地上半寄生现象基因组基础的理想模式材料。本研究报道了基于PacBio高精度（HiFi）测序与Hi-C辅助组装技术获得的广寄生高质量染色体级基因组组装结果：组装完成的基因组总长度为547.41 Mb，contig N50达到8.32 Mb，其中97.54%的序列可锚定至9条假染色体上；重复序列占基因组总长度的64.53%。本研究共预测得到21837个蛋白质编码基因，其中96.05%的基因获得了功能注释。值得注意的是，通过PlantTFDB数据库共鉴定得到1271个转录因子基因。此外，本研究共注释得到8407个非编码RNA，其中包括3821个转运RNA（tRNA）、3076个小核RNA（snRNA）、1447个核糖体RNA（rRNA）以及63个微小RNA（miRNA）。本研究通过BUSCO评估、LTR组装指数（LTR assembly index）、Illumina测序读段比对以及端粒重复序列检测等多种方法对基因组组装质量进行验证，所有结果均证实该组装具有极高的完整性与准确性。该染色体级基因组组装为研究广寄生的基因家族演化、寄生适应性以及基因组结构提供了重要的基础资源。该基因组及相关数据集已公开共享，可为未来寄生被子植物的比较基因组学与功能基因组学研究提供支撑。