Tissue distribution of <i>Wolbachia</i> in <i>Cubitermes</i> castes and stages.

Queens were from T5 (C. spA) and T42 (C. spB), kings were from T38 (C. spA) and T42 (C. spB), workers, white soldiers and mature soldiers were from T42 (C. spB) and workers’ salivary glands were from T34 (C. sp A) and T42 (C. spB). The occurrence of Wolbachia was tested using wsp PCR and recorded as positive (+) or negative (-). The DNA quality was tested by using 12S ribosomal RNA gene. Tissue distribution of Wolbachia in Cubitermes castes and stages.蚁后取自T5品系（弯颚白蚁属物种A，Cubitermes sp. A）与T42品系（弯颚白蚁属物种B，Cubitermes sp. B）；雄蚁取自T38品系（C. spA）与T42品系（C. spB）；工蚁、白兵蚁与成熟兵蚁取自T42品系（C. spB）；工蚁唾液腺则取自T34品系（C. spA）与T42品系（C. spB）。 采用wsp聚合酶链式反应（wsp PCR）检测沃尔巴克氏体（Wolbachia）的感染情况，结果记为阳性（+）或阴性（-）。 通过12S核糖体RNA基因（12S ribosomal RNA gene）检测DNA样本质量。 沃尔巴克氏体在弯颚白蚁社会品级及发育阶段中的组织分布