Proconvulsants increase synchronicity of neuron activity.

A) GCaMP5 fluorescence from single aCC neurons from consecutive segments show hyperactive and synchronised pattern changes following 4 min application of 4-AP (3 mM). Dotted line shows period when 4-AP was perfused and downward arrow indicates time 4-AP entered the bath. The time difference results from the length of tubing between gravity-driven perfusion system and the recording chamber. B) PTX (5μM) perfusion similarly increases neuron activity. Dotted line shows period when PTX was perfused and downward arrow indicates time PTX entered the bath. C, D) Analysis of number of synchronised aCC cells before and after 4-AP (C) and PTX (D) application (values are mean ± sem for n = 6 and 5, respectively). Synchrony was compared between a fixed ROI (labelled brown in the inset) and compared to all ROIs (red box on inset) and with those on the ipsilateral (green box) or contralateral (blue box) sides.

A) 来自连续节段的单个aCC神经元的GCaMP5荧光信号，在施加3 mM的4-AP并持续4分钟后，呈现出活动过度且同步化的模式改变。虚线标注4-AP的灌流时段，向下箭头指示4-AP流入记录浴槽的时刻。该时间差由重力驱动灌流系统与记录浴槽之间的管路长度所导致。
B) 5 μM的PTX灌流同样可增强神经元活动。虚线标注PTX的灌流时段，向下箭头指示PTX流入记录浴槽的时刻。
C、D) 分别对施加4-AP（C组）与PTX（D组）前后的同步化aCC细胞数量进行分析（样本量n分别为6和5，数值以均值±标准误（standard error of the mean, SEM）表示）。同步性的比较以固定的感兴趣区域（Region of Interest, ROI，插图中标注为棕色）为参照，分别与全部感兴趣区域（插图中红色框）、同侧（插图中绿色框）及对侧（插图中蓝色框）的感兴趣区域进行对比。