Influence of cultivar, fungal exudates fractions and zinniol on cell suspension esterase activity.

Carrot cell suspensions with six different genotypes were tested for esterase specific activity in the presence of fungal extracts and toxins.1ESA is for Esterase Specific Activity, expressed in nmol min−1 mg(prot)−1.2Activities with the same letter are not significantly different. h1 homogeneity groups were obtained by a single ANOVA analysis of all the results followed by LSD multiple comparisons.3Activities with the same letter are not significantly different. h2 homogeneity groups were obtained by a separate ANOVA analysis of the results for each cultivar followed by LSD multiple comparisons.4The treatments were as follows: rA: Alternaria dauci (strain FRA017) fungal culture raw extract; rM: uninoculated medium raw extract; aA: A. dauci fungal culture aqueous extract; aM: uninoculated medium aqueous extract; oA: A. dauci fungal culture organic extract; oM: uninoculated medium organic extract; DMSO: DMSO solution at a concentration corresponding to oM, z1, z2 and z3 treatments; z1: 0.025 µM zinniol; z2: 10 µM zinniol; z3: 500 µM zinniol. C: no treatment.本实验针对6种不同基因型的胡萝卜细胞悬浮系，检测其在真菌提取物与毒素共存条件下的酯酶比活性（Esterase Specific Activity，ESA）。1 酯酶比活性的计量单位为nmol·min⁻¹·mg(prot)⁻¹。2 带有相同字母的活性值无显著差异：h1同质子集是通过对全部实验结果进行单因素方差分析（Analysis of Variance，ANOVA）后，结合最小显著差异（Least Significant Difference，LSD）多重比较得到的。3 带有相同字母的活性值无显著差异：h2同质子集是通过对每个栽培品种的实验结果分别开展单因素方差分析，再辅以最小显著差异多重比较得到的。4 各处理组设置如下：rA为早枯链格孢（Alternaria dauci，菌株FRA017）的真菌培养物粗提取物；rM为未接种的培养基粗提取物；aA为早枯链格孢（A. dauci）真菌培养物水提取物；aM为未接种的培养基水提取物；oA为早枯链格孢（A. dauci）真菌培养物有机溶剂提取物；oM为未接种的培养基有机溶剂提取物；DMSO为浓度与oM处理组匹配的二甲基亚砜溶液，对应z1、z2、z3处理组；z1为0.025 μM的百日菊素（zinniol）；z2为10 μM的百日菊素；z3为500 μM的百日菊素。C：无处理组。