Comparison of Thermoanaerobacter sp. X514 grown on different xylose concentrations. Thermoanaerobacter sp. X514

Thermoanaerobacter sp. X514 was grown on 1, 10, 50 and 200 mM xylose in order to identify pathways and transporters responsible for xylose transport and conversion to ethanol. Overall design: In this study, X514 cells were grown in difeined medium supplemented with 1, 10, 50 or 200 mM xylose as the sole carbon substrate. Each sample was harvested at mid-log phase. Each sample was run in triplicate using genomic DNA as the control.

为鉴定介导木糖转运及乙醇生成的代谢通路与转运蛋白，本研究以1 mM、10 mM、50 mM及200 mM木糖为唯一碳源，培养热厌氧杆菌属（Thermoanaerobacter sp.）菌株X514。
整体实验设计：本研究中，X514菌株在化学成分确定培养基（defined medium）中培养，以1 mM、10 mM、50 mM或200 mM木糖作为唯一碳源底物。所有样本均于对数中期收获，每组均以基因组DNA作为对照并设置三次生物学重复。