Expression and production of CSF-1 in breast cancer cells.

ELISA of cell culture supernatants with an antibody directed to biologically-active human CSF-1; −: below assay sensitivity; +: 50 pg/ml; nd: not determined. WB: western blotting of cell lysates and supernatants using a goat polyclonal antibody (N-16). +, ++, +++ refer to the presence of detectable, marked or large amounts of CSF-1-specific bands, respectively.采用针对具有生物学活性的人集落刺激因子1（CSF-1）的抗体，对细胞培养上清液进行酶联免疫吸附实验（ELISA）；−：低于检测灵敏度；+：对应浓度为50 pg/ml；nd：未测定。蛋白质免疫印迹（Western Blot，WB）：采用山羊多克隆抗体（N-16）对细胞裂解液及上清液进行检测；+、++、+++分别代表可检测到、显著或大量的CSF-1特异性条带。