Decorating chromatin for enhanced genome editing using CRISPR-Cas9

To improve the efficiency of homology-directed repair of double-stranded breaks introduced by CRISPR-Cas9, a Cas9-PRDM9 (PR/SET Domain 9) fusion protein was generated and tested in HEK-293T cells. RNA-seq was used to quantify gene expression in the parental HEK-293T cell line.为提升CRISPR-Cas9介导的双链断裂（double-stranded breaks, DSBs）的同源定向修复（homology-directed repair, HDR）效率，本研究构建了Cas9-PRDM9（PR/SET结构域9, PR/SET Domain 9）融合蛋白，并在HEK-293T细胞中进行了实验测试。同时，采用RNA测序（RNA-seq）对亲本HEK-293T细胞系的基因表达水平开展定量分析。