ALDH1L1 bacTRAP microarray from striatum 12, 24, and 48 hrs post-MPTP exposure (mice)

Raw data from Illumina MouseRef-8 v2 Expression BeadChip microarray. ALDH1L1 bacTRAP mice were exposed to a single dose of MPTP (12.5 mg/kg, s.c.) or saline and sacrificed at 12, 24, or 48 hours post-exposure. Astrocyte RNA was isolated from pooled striatal tissue (7 mice/sample; 2-3 samples per group) following the TRAP procedure [Heiman et al. (2014) Cell type-specific mRNA purification by translating ribosome affinity purification (TRAP). Nat Protoc, 9: 1292-1291.]

本数据集来源于Illumina MouseRef-8 v2表达谱微珠芯片的原始检测数据。实验所用动物为ALDH1L1 bacTRAP小鼠，分别给予单次皮下注射剂量为12.5 mg/kg的1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）或生理盐水，并于给药后12、24或48小时实施安乐死。后续采用TRAP（translating ribosome affinity purification，翻译核糖体亲和纯化）实验流程，从合并的纹状体组织中分离星形胶质细胞RNA，每样本使用7只小鼠，每组设置2-3个重复样本。相关实验方法引自：Heiman等(2014)，《通过翻译核糖体亲和纯化（TRAP）实现细胞类型特异性mRNA纯化》，Nature Protocols（Nat Protoc），9: 1292-1291。