Gene expression profile at single cell level of small intestine epithelial cells from the distal ileum

Small intestine is highly innervated by nociceptor neurons. Single cell RNA sequencing (scRNA-seq) of ileal epithelial cells was performed to analyze the role of nociceptor neurons in regulating small intestinal epithelial cells. Ileal epithelial cells were purified by Fluorescence-activated cell sorting (FACS) from both nociceptor knockout (Nav1.8Cre_P_DTA_P) mice and the control (Nav1.8Cre_M_DTA_P) mice. The purified cells were used for scRNAseq using inDrops.

小肠受到伤害感受神经元（nociceptor neurons）的高度神经支配。为解析伤害感受神经元对小肠上皮细胞的调控作用，研究人员针对回肠上皮细胞开展了单细胞RNA测序（scRNA-seq）。研究人员分别从伤害感受神经元敲除（Nav1.8Cre_P_DTA_P）小鼠及对照（Nav1.8Cre_M_DTA_P）小鼠体内，通过荧光激活细胞分选（FACS）纯化得到回肠上皮细胞，随后利用inDrops平台对纯化后的细胞进行scRNA-seq测序。