Genome-wide analysis of distribution of RNA Pol II ChIP-seq in NKAP depletion cells. Genome-wide analysis of distribution of RNA Pol II ChIP-seq in NKAP depletion cells

We performed ChIP-Seq analysis with an antibody against RNA polymerase II (Pol II). We found that the distribution of Pol II signals was decreased at the transcription start sites (TSSs) and transcription end sites (TESs) upon depletion of NKAP. Overall design: Chromatin Immunoprecipitation DNA-Sequencing (ChIP-seq) to analyze RNA polymerase II in U2OS cells that were transfected with two different types of small interfering RNAs (siRNAs), namely NC and NKAP_KD.

本研究使用针对RNA聚合酶II（RNA polymerase II, Pol II）的抗体开展了染色质免疫共沉淀DNA测序（Chromatin Immunoprecipitation DNA-Sequencing, ChIP-Seq）分析。研究发现，在NKAP被敲低后，Pol II信号在转录起始位点（transcription start sites, TSSs）与转录终止位点（transcription end sites, TESs）处的分布出现下降。
总体实验设计：采用染色质免疫共沉淀DNA测序（ChIP-seq）分析转染两种不同小干扰RNA（small interfering RNAs, siRNAs，即阴性对照NC与NKAP敲低组NKAP_KD）的U2OS细胞中的RNA聚合酶II。