Phosphorylation of CPSF6 RS-like domain

Cleavage factor I mammalian (CFIm) complex, composed of cleavage and polyadenylation specificity factor CPSF6, regulates alternative polyadenylation (APA). CPSF6 has a RS-like domain which plays role in protein -protein interactions. This interaction might have role in alternative polyadenylation site selection. The phosphorylation of RS- like domain might play role in protein -protein interaction and thus might have a role in alternative polyadenylation site selection. So we did mass specteroanalysis to analyse phosphorylation sites of RS-like domain of CPSF6.

哺乳动物切割因子I（Cleavage factor I mammalian, CFIm）复合物由切割与多聚腺苷酸化特异性因子CPSF6组成，可调控可变多聚腺苷酸化（alternative polyadenylation, APA）。CPSF6含有类精氨酸-丝氨酸结构域（RS-like domain），该结构域在蛋白质-蛋白质相互作用中发挥功能；此类相互作用可能参与可变多聚腺苷酸化位点的选择过程。类RS结构域的磷酸化修饰，同样可能通过影响蛋白质-蛋白质相互作用，进而在可变多聚腺苷酸化位点选择中发挥作用。基于此，我们开展了质谱分析，以解析CPSF6的类RS结构域的磷酸化位点。