Alignments of 18S,5.8S and 26S rDNA sequences of plants and fungal

The alignments were constructed using 18S, 5.8S and 26S rDNA sequences of all plants and fungal from GenBank on 9th February 2013. Multiple alignments of every group were conducted using MAFFT v6.864 (Katoh et al. 2005). The regions from NS7 priming site to the 3’ end of 18S, the 5’ end to LR3 priming site of 26S, and the complete 5.8S region were extracted for primer design and in silico tests. The "NonGreen plants" includes Rhodophytes and Glaucophytes. The "Basal fungi" includes Microsporidia and Cryptomycota.

本研究构建的多序列比对基于2013年2月9日从GenBank数据库获取的所有植物与真菌的18S、5.8S和26S核糖体DNA（rDNA）序列。所有类群的多序列比对均采用MAFFT v6.864软件（Katoh等，2005）完成。研究提取了18S rDNA从NS7引物结合位点至其3'端、26S rDNA从其5'端至LR3引物结合位点的区域，以及完整的5.8S rDNA区域，用于引物设计与计算机模拟（in silico）测试。"非绿色植物（NonGreen plants）"类群包含红藻门（Rhodophytes）与灰藻门（Glaucophytes）。"基部真菌（Basal fungi）"类群包含微孢子虫门（Microsporidia）与隐真菌门（Cryptomycota）。