IWGSC Chromosome sequence survey of hexaploid wheat CS42

An international project coordinated by the International Wheat Genome Sequencing Consortium (IWGSC, http://www.wheatgenome.org) to generate sequence reads from each of the 21 individual chromosomes of hexaploid wheat (Chinese Spring line 42). Illumina sequence reads for individual chromosome arms were generated from amplified DNA prepared by flow-sorting chromosomes from aneuploid wheat lines. Sequencing libraries were prepared from sheared 500bp fragments and sequenced on Hi-Seq or GAIIX instruments to produce 100 or 150bp paired reads. Reads were assembled with ABySS to produce a set of contigs for each of the chromosomes that have been used to assess a large part of the gene content of each chromosome and to derive a virtual gene order based on comparative genomics and colinearity with other grass genomes. IWGSC data repository can be accessed here: http://wheat-urgi.versailles.inra.fr/Seq-Repository.

本项目由国际小麦基因组测序联盟（International Wheat Genome Sequencing Consortium, IWGSC，http://www.wheatgenome.org）协调，旨在对六倍体小麦（中国春品系42）的全部21条独立染色体分别生成测序读段。研究人员从非整倍体小麦品系中流式分选染色体，制备扩增后的DNA，进而得到各染色体臂的Illumina测序读段。测序文库以剪切为500bp的DNA片段构建，并在Hi-Seq或GAIIX测序仪上完成测序，产出100bp或150bp的双端读段。使用ABySS对测序读段进行组装，得到每条染色体的重叠群（contigs）集合；该集合已被用于评估各染色体的绝大多数基因组成分，并基于比较基因组学以及与其他禾本科基因组的共线性关系，推导得到虚拟基因顺序。可通过以下链接访问IWGSC数据仓库：http://wheat-urgi.versailles.inra.fr/Seq-Repository。