Analysis of the differential expression profile of miRNAs in myocardial tissues of rats with burn injury

Fifteen percent third-degree burn rat model was used to identify miRNAs that are markers of burn injury-induced myocardial damage. Cardiac tissues were evaluated to determine miRNA profile sequencing. Pearson’s correlation analysis was used between miRNAs and injury markers. ROC curve analysis was used to estimate miRNA’s sensitivity and specificity for the diagnosis of myocardial damage caused by burn injury. The sequencing analysis revealed 23 differentially expressed miRNAs. Pearson’s correlation analysis revealed that rno-miR-190b-3p and C5b9, rno-miR-341, rno-miR-344b-3p and TnI, rno-miR-344b-3p and CK-MB were significantly positively correlated, respectively. ROC curve analysis demonstrated that rno-miR-341, rno-miR-344b-3p, and rno-miR-190b-3p exhibited high sensitivity and specificity for the diagnosis of myocardial damage caused by burn injury. In conclusion, our results suggest that rno-miR-341, rno-miR-344b-3p, and rno-miR-190b-3p have the potential to be used as sensitive and specific biomarkers to diagnose myocardial damage caused by burn injury. Validation of candidate miRNAs and correlation analysis Validation of candidate miRNAs and correlation analysis

本研究采用15%体表面积Ⅲ度烧伤大鼠模型，筛选可作为烧伤诱导心肌损伤标志物的微小RNA（microRNAs，miRNAs）。获取大鼠心肌组织并开展miRNA表达谱测序分析。采用Pearson相关分析探究miRNAs与心肌损伤标志物间的相关性，并通过受试者工作特征（Receiver Operating Characteristic, ROC）曲线分析评估miRNAs对烧伤诱导心肌损伤的诊断灵敏度与特异度。测序分析共筛选得到23个差异表达miRNAs。Pearson相关分析结果显示，rno-miR-190b-3p与C5b-9、rno-miR-341、rno-miR-344b-3p与肌钙蛋白I（Troponin I, TnI）、rno-miR-344b-3p与肌酸激酶同工酶MB（Creatine Kinase MB, CK-MB）均呈显著正相关。ROC曲线分析表明，rno-miR-341、rno-miR-344b-3p及rno-miR-190b-3p对烧伤诱导心肌损伤具有较高的诊断灵敏度与特异度。综上，本研究结果表明rno-miR-341、rno-miR-344b-3p及rno-miR-190b-3p有望成为诊断烧伤诱导心肌损伤的灵敏且特异的生物标志物。候选miRNA验证及相关性分析 候选miRNA验证及相关性分析