Direct and indirect podocyte injury manifested in a partial podocytectomy mouse model

Injured podocytes indirectly influence neighboring podocytes. To study the indirect effect, we generated mosaic mice, Pod-hCD25T/G, in which podocytes express either hCD25 + tdTomato or EGFP alone. A hCD25-targetting immunotoxin LMB2 was injected into the mosaic mice to induce injury selectively in tdTomato(+) podocytes. We analyzed RNA expressing profile in tdTomato(+) (hCD25 expressing) and EGFP(+) (hCD25 not expressing) podocytes before and after LMB2 injection. Glomeruli were harvested from Pod-hCD25T/G mouse before and 1, 4, and 7 days after LBM2 treatment. tdTomato(+) and EGFP(+) podocytes were collected by cell sorter and RNA expressing profile was analyzed.

受损的足细胞会间接影响邻近的足细胞。为研究该间接效应，我们构建了嵌合小鼠Pod-hCD25T/G，该小鼠的足细胞仅表达hCD25+tdTomato或仅表达EGFP。我们向该嵌合小鼠体内注射靶向hCD25的免疫毒素LMB2，以选择性诱导tdTomato阳性足细胞发生损伤。我们分别在LMB2注射前后，对tdTomato阳性（表达hCD25）与EGFP阳性（不表达hCD25）的足细胞进行RNA表达谱分析。我们在LMB2处理前以及处理后1、4、7天，从Pod-hCD25T/G小鼠体内收集肾小球。通过细胞分选仪分离得到tdTomato阳性与EGFP阳性足细胞，并对其RNA表达谱进行检测分析。