Sequence diversity in MAX effectors and other genes in 120 isolates of the rice blast fungus Magnaporthe oryzae
收藏资源简介:
- list_of_accessions_and_assembly_statistics.xlsx: list of 120 isolate and genome assembly statistics - assemblies.zip: genome assemblies with repeats were not masked - orthogroups.txt: list of orthogroups in orthogroups.zip -orthogroups.zip: sequences of orthogroups, as identified using Orthofinder. Sequences were aligned using translatorX (https://doi.org/10.1093/nar/gkq291) - single_copy_orthologs.zip: folder which contains aligned sequences of single-copy orthologs (alignment with translatorX https://doi.org/10.1093/nar/gkq291); three types of genes were distinguished: MAX effectors, other secreted proteins, and other genes; note that to produce this dataset, the 11 orthogroups that included paralogous copies of MAX effectors were split into sets of orthologous sequences using genealogies inferred using RAXML v8, yielding a total of 94 single-copy MAX orthologs; for each split orthogroup, sets of orthologous sequences were assigned a number that was added to the orthogroup’s identifier as a suffix (for instance paralogous sequences of orthogroup OG0000244 were split into orthogroups OG0000244_1 and OG0000244_2)
- list_of_accessions_and_assembly_statistics.xlsx:收录120个分离株及其基因组组装统计信息的文件 - assemblies.zip:未进行重复序列屏蔽的基因组组装结果压缩包 - orthogroups.txt:orthogroups.zip内的直系同源组(orthogroup)列表 - orthogroups.zip:采用Orthofinder鉴定得到的直系同源组(orthogroup)序列压缩包。序列通过translatorX(https://doi.org/10.1093/nar/gkq291)完成多序列比对 - single_copy_orthologs.zip:包含单拷贝直系同源基因比对序列的文件夹,比对工具同样为translatorX(https://doi.org/10.1093/nar/gkq291);该数据集划分了三类基因:MAX效应蛋白、其他分泌蛋白及其他基因;需特别说明,为构建本数据集,研究人员将包含MAX效应蛋白旁系同源拷贝的11个直系同源组,通过RAXML v8推断的系统发育谱系拆分为直系同源序列集,最终得到94个单拷贝MAX直系同源基因;对于每个拆分后的直系同源组,其直系同源序列集会被分配一个编号作为该组标识符的后缀(例如,直系同源组OG0000244的旁系同源序列被拆分为OG0000244_1与OG0000244_2)



