Additional file 7: of A comparison of heat-stress transcriptome changes between wild-type Arabidopsis pollen and a heat-sensitive mutant harboring a knockout of cyclic nucleotide-gated cation channel 16 (cngc16)
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https://springernature.figshare.com/articles/Additional_file_7_of_A_comparison_of_heat-stress_transcriptome_changes_between_wild-type_Arabidopsis_pollen_and_a_heat-sensitive_mutant_harboring_a_knockout_of_cyclic_nucleotide-gated_cation_channel_16_cngc16_/6858929/1
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RNA-Seq validation using real-time Q-PCR. a. Comparison of expression values obtained from Q-PCR and RNA-Seq normalized to WT control (normal). The analysis was performed on two different reference genes separately (CYCP2 (AT3G21870) and UBQ7 (AT2G35635)). b. Primer sequences used for real-time Q-PCR. (XLSX 16 kb)
基于实时定量聚合酶链反应（real-time Q-PCR）的RNA测序（RNA-Seq）验证数据集。
a. 对比以野生型（Wild Type）对照（正常组）为参照进行标准化处理后，实时定量PCR与RNA测序所得的基因表达量。本次分析分别针对两个内参基因开展：CYCP2（AT3G21870）与UBQ7（AT2G35635）。
b. 实时定量PCR所用引物序列。（XLSX格式，文件大小16 KB）
提供机构：
Chrystle Weigand
创建时间：
2018-07-25
