DataSheet_1_Complete chloroplast genome sequences of Phlomis fruticosa and Phlomoides strigosa and comparative analysis of the genus Phlomis sensu lato (Lamiaceae).fasta

The taxonomic terms “Phlomis” and “Phlomoides” had been used to describe two sections within the genus Phlomis belonging to the Lamiaceae family. Recently, phylogenetic analyses using molecular markers showed that Phlomis and Phlomoides formed two monophyletic clades, and thus they are generally accepted as separate genera. In this study, we assembled the complete chloroplast genome of Phlomis fruticosa, which is the first reported chloroplast genome belonging to Phlomis genus, as well as the complete chloroplast genome of Phlomoides strigosa belonging to Phlomoides genus. The results showed that the length of chloroplast genome was 151,639 bp (Phlomis fruticosa) and 152,432 bp (Phlomoides strigosa), with conserved large single copy regions, small single copy regions, and inverted repeat regions. 121 genes in Phlomis fruticosa and 120 genes in Phlomoides strigosa were annotated. The chloroplast genomes of Phlomis fruticosa, Phlomoides strigosa, and three reported Phlomoides species, as well as those of 51 species from the Lamiaceae family, which covered 12 subfamilies, were subjected to phylogenetic analyses. The Phlomis and Phlomoides species were split into two groups, which were well supported by both maximum likelihood and Bayesian inference tree analyses. Our study provided further evidence to recognize Phlomis and Phlomoides as independent genera.

分类学术语“糙苏属（Phlomis）”与“假糙苏属（Phlomoides）”曾被用于指代唇形科（Lamiaceae）糙苏属（Phlomis）下的两个分类组。近期，基于分子标记的系统发育分析显示，糙苏属与假糙苏属各自形成单系支，因此二者现已被普遍接受为独立的属。本研究组装了灌木糙苏（Phlomis fruticosa）的完整叶绿体基因组（chloroplast genome），这是首个被报道的糙苏属叶绿体基因组；同时还组装了刚毛假糙苏（Phlomoides strigosa）的完整叶绿体基因组。结果表明，灌木糙苏与刚毛假糙苏的叶绿体基因组长度分别为151639 bp与152432 bp，二者均包含保守的大单拷贝区、小单拷贝区以及反向重复区。其中，灌木糙苏共注释得到121个基因，刚毛假糙苏共注释得到120个基因。本研究将灌木糙苏、刚毛假糙苏、已报道的3个假糙苏属物种，以及覆盖12个亚科的51个唇形科物种的叶绿体基因组纳入系统发育分析。结果显示，糙苏属与假糙苏属物种被划分为两个类群，该分类结果得到最大似然法与贝叶斯推断树分析的强力支持。本研究为将糙苏属与假糙苏属认定为独立属提供了进一步的实验证据。