Data_Sheet_5_The First Mitochondrial Genome of Ciborinia camelliae and Its Position in the Sclerotiniaceae Family.docx

Ciborinia camelliae is the causal agent of camellia flower blight (CFB). It is a hemibiotrophic pathogen, inoperculate Discomycete of the family Sclerotiniaceae. It shows host and organ specificity infecting only flowers of species belonging to the genus Camellia, causing serious damage to the ornamental component of the plant. In this work, the first mitochondrial genome of Ciborinia camellia is reported. The mitogenome was obtained by combining Illumina short read and Nanopore long read technology. To resolve repetitive elements, specific primers were designed and used for Sanger sequencing. The manually curated mitochondrial DNA (mtDNA) of the Italian strain DSM 112729 is a circular sequence of 114,660 bp, with 29.6% of GC content. It contains two ribosomal RNA genes, 33 transfer RNAs, one RNase P gene, and 62 protein-coding genes. The latter include one gene coding for a ribosomal protein (rps3) and the 14 typical proteins involved in the oxidative metabolism. Moreover, a partial mtDNA assembled from a contig list was obtained from the deposited genome assembly of a New Zealand strain of C. camelliae. The present study contributes to understanding the mitogenome arrangement and the evolution of this phytopathogenic fungus in comparison to other Sclerotiniaceae species and confirms the usefulness of mitochondrial analysis to define phylogenetic positioning of this newly sequenced species.

山茶生核盘菌（Ciborinia camelliae）是山茶花卉枯病（CFB）的致病菌。该菌为半活体营养型致病菌（hemibiotrophic pathogen），隶属于核盘菌科（Sclerotiniaceae）的无囊盖盘菌（inoperculate Discomycete）类群。其具有寄主与器官专一性，仅侵染山茶属（Camellia）植物的花器，对该植物的观赏价值造成严重损害。本研究首次报道了山茶生核盘菌的线粒体基因组（mitochondrial genome）。该线粒体基因组通过结合Illumina短读长测序（Illumina short read）与Nanopore长读长测序（Nanopore long read）技术获取。为解析重复序列区域，研究人员设计了特异性引物并用于桑格测序（Sanger sequencing）。经人工整理注释的意大利菌株DSM 112729的线粒体DNA（mtDNA）为环状序列，全长114,660 bp，GC含量为29.6%。该线粒体基因组包含2个核糖体RNA基因（ribosomal RNA genes）、33个转运RNA基因（transfer RNAs）、1个核糖核酸酶P（RNase P）基因以及62个蛋白质编码基因（protein-coding genes）。其中蛋白质编码基因包含1个核糖体蛋白编码基因（rps3）与14种参与氧化代谢的典型功能蛋白。此外，研究人员从已提交的新西兰山茶生核盘菌菌株的基因组组装结果中，通过重叠群（contig）列表组装得到一段部分线粒体基因组序列。本研究通过与其他核盘菌科物种进行比较分析，有助于阐明该植物致病真菌的线粒体基因组结构与演化历程，并证实了线粒体分析可用于明确该新测序物种的系统发育定位（phylogenetic positioning）。