Smart-Seq of male blastocysts from mothers bearing the OgtT931A mutation.

40-bp PE mRNA-Seq of single E4 blastocysts generated through IVF of oocytes harvested from two F0 OgtT931A/WT females and from two littermate WT females, using the same WT sperm. Sex was established by PCR genotyping of the cDNA and only male blastocysts were sequenced. One sample (het_1A) is a female (deduced in silico from the sequencing data) but it is present because of incorrect PCR genotyping. Presence of the mutation among the embryos from the OgtT931A/WT females was also enstablished from the cDNA via PCR genotyping and Sanger sequencing - only two hemizygous OgtT931A/Y embryos were found (het_3D, het_5G).

本数据集源自对两类雌性小鼠的卵母细胞经体外受精（IVF）后获得的单个E4期囊胚的40碱基对双端mRNA测序（40-bp PE mRNA-Seq）：一类为两只F0代OgtT931A/WT雌性小鼠，另一类为两只同窝野生型（WT）雌性小鼠，两组实验均使用同一批野生型精子。实验通过对互补脱氧核糖核酸（cDNA）进行聚合酶链式反应（PCR）基因分型确定囊胚性别，仅对雄性囊胚开展测序。其中样本het_1A经测序数据的生物信息学推导为雌性，该样本因PCR基因分型错误被纳入本次数据集。OgtT931A/WT雌性小鼠所产胚胎中该突变的存在性，亦通过cDNA的PCR基因分型与桑格测序（Sanger sequencing）得到验证，本次实验仅发现两只半合子OgtT931A/Y胚胎（het_3D、het_5G）。