Copaiba oleoresin presents anti-obesogenic effect and mitigates inflammation and redox imbalance in adipose tissue

ABSTRACT Obesogenic diets lead to fat accumulation and dysfunctional adipose tissue. Active compounds were described in copaiba oleoresin, which presents anti-inflammatory, antimicrobial, and antioxidant properties. However, there are no data regarding the effects of copaiba oleoresin in adipose tissue. Therefore, we tested the hypothesis that the copaiba oleoresin could prevent or minimize obesity and adipose tissue inflammation and oxidative stress in response to a high sucrose diet. Male Wistar rats were randomly assigned to receiving commercial chow (C, n = 8), commercial chow and 30% sucrose added to the drinking water (S, n = 8), or commercial chow and 30% sucrose added to the drinking water + copaiba oleoresin (S+CO, n = 8). Copaiba oleoresin was given at a dose of 200 mg kg-1 day-1 by gavage for eight weeks. C and S animals received vehicle, at equivalent volume, by gavage. At the end of the experiment, blood samples and epididymal adipose tissue were collected for biochemical, inflammatory, and oxidative stress analyses. Copaiba oleoresin supplementation prevented weight gain, adiposity, insulin resistance, and increased IL-1β levels. Additionally, copaiba oleoresin partially attenuated the increase in fasting glucose levels, lipids, and IL-6 levels, and improved the redox status in adipose tissue. Our results suggest that the use of copaiba oleoresin could be a good strategy for prevention of obesity and its complications.

摘要：致肥胖饮食可引发脂肪堆积与脂肪组织功能障碍。古巴香脂油树脂（copaiba oleoresin）中含有多种活性成分，具备抗炎、抗菌及抗氧化特性。但目前尚无关于古巴香脂油树脂对脂肪组织影响的相关研究数据。因此，本研究验证如下假说：古巴香脂油树脂可在高蔗糖饮食干预中，预防或减轻肥胖、脂肪组织炎症及氧化应激反应。将雄性Wistar大鼠随机分为3组：普通饲料组（C组，n=8）、普通饲料+饮用水添加30%蔗糖组（S组，n=8），以及普通饲料+饮用水添加30%蔗糖+古巴香脂油树脂组（S+CO组，n=8）。古巴香脂油树脂以200 mg·kg⁻¹·d⁻¹的剂量通过灌胃给药，持续8周；C组与S组大鼠则灌胃给予等体积的溶剂对照。实验结束后，采集血液样本与附睾脂肪组织，用于生化、炎症及氧化应激相关指标分析。结果显示，古巴香脂油树脂补充干预可阻止体重增加、脂肪蓄积与胰岛素抵抗，同时降低IL-1β水平。此外，古巴香脂油树脂可部分缓解空腹血糖、血脂及IL-6水平的升高，并改善脂肪组织的氧化还原状态。本研究结果表明，古巴香脂油树脂或可作为预防肥胖及其并发症的有效策略。