Gene expression profiles in the cultured dorsal root ganglia (DRG) neurons treated with PBS or oncomodulin (ONCM). Gene expression profiles in the cultured dorsal root ganglia (DRG) neurons treated with PBS or oncomodulin (ONCM)

Preconditioning nerve injury drives pro-regenerative perineuronal macrophage activation in dorsal root ganglia (DRG). The present study reports that oncomodulin (ONCM) is produced from the regeneration-associated macrophages (RAMs) and strongly influences regeneration of DRG sensory axons. ONCM in macrophages was necessary to produce RAMs in the in vitro model of neuron-macrophage interaction and played an essential role in for preconditioning-induced neurite outgrowth. In order to gain insight on potential mechanisms downstream of ONCM for potent neurite outgrowth activity, we performed RNA-seq using cultured DRG neurons treated with ONCM. Overall design: Cultured DRGs neurons from WT mouse and treated with PBS or 100 ng/ml of recombinant ONCM for 12 hr.

预处理性神经损伤可诱导背根神经节（dorsal root ganglia, DRG）内促再生的神经元周围巨噬细胞活化。本研究报道，癌调蛋白（oncomodulin, ONCM）由再生相关巨噬细胞（regeneration-associated macrophages, RAMs）分泌，并对背根神经节感觉轴突的再生具有显著调控作用。在神经元-巨噬细胞互作的体外模型中，巨噬细胞来源的ONCM是RAMs生成的必要条件，同时在预处理诱导的神经突生长过程中发挥不可或缺的作用。为深入解析ONCM介导强效神经突生长活性的潜在下游调控机制，我们采用经ONCM处理的体外培养背根神经节神经元开展了RNA测序（RNA-seq）。实验整体设计：取自野生型（wild type, WT）小鼠的培养背根神经节神经元经磷酸盐缓冲液（phosphate-buffered saline, PBS）或100 ng/ml重组ONCM处理12小时。