Identification of genes dependent on the Ecdysone receptor (EcR) at the onset of metamorphosis in Drosophila. Drosophila melanogaster

This study identifies those genes that are dependent on EcR for their proper regulation at the onset of metamorphosis in Drosophila melanogaster. Keywords: Nuclear receptor gene regulation Overall design: hs-EcRi-11 third instar larvae were heat-treated twice at 37°C, each time for 1 hour, at 24 hours and 18 hours prior to pupariation, as described [29]. RNA was harvested for microarray analysis at -4, 0, and 4 hours relative to pupariation from three independent collections of animals for each time point. Data obtained from these arrays was compared to array data from control animals at the same stages of development to identify EcR-dependent genes.

本研究鉴定了黑腹果蝇（Drosophila melanogaster）变态起始阶段中，正常调控依赖于蜕皮激素受体（EcR）的基因。关键词：核受体基因调控。实验设计：将hs-EcRi-11三龄幼虫在化蛹前24小时与18小时，于37℃下进行两次热激处理，每次时长1小时，具体操作参照文献[29]。在相对于化蛹的-4小时、0小时及4小时三个时间点，针对每个时间点均从三个独立的动物样本中提取RNA，用于芯片分析。将本次芯片实验所得数据与相同发育阶段对照组动物的芯片数据进行比对，以此鉴定依赖于EcR的基因。