Surface Topography Enhances Differentiation of Mesenchymal Stem Cells Towards Osteogenic and Adipogenic Lineages. Homo sapiens

Surface topography impacts on cell growth and differentiation, but it is not trivial to generate homogeneous surface structures and to define the specific morphological parameters of relevance. In this study, we have compared gene expression profiles of mesenchymal stem cells (MSCs) on nanostructured groove/ridge surfaces. Patterns were generated in polyimide using multi beam laser interference. These structures affected cell size and orientation of human MSCs. Furthermore, the nano-patterns with a periodicity of 650 nm increased differentiation towards osteogenic and adipogenic lineages. However, in absence of differentiation media the surface structures did neither induce differentiation, nor lineage-specific gene expression changes – as assessed by genome wide gene expression profiles with Affymetrix microarray technology. Our results demonstrate that grooves and ridges at a periodicity of 650 nm enhance the propensity of MSCs to differentiate towards adipogenic and/or osteogenic lineages – but they do not directly govern lineage-specific gene expression changes. Overall design: 9 samples were hybridized to the GeneChip Human Gene 2.0 ST Array (Affymetrix). In comparison to the manuscript the donor IDs refer as follows: donor 1 = AT57; donor 2 = AT58; donor 3 = AT61.

表面形貌可影响细胞的生长与分化，但构建均一的表面结构并明确与之相关的特异性形态学参数并非易事。本研究针对纳米结构沟槽/脊表面的间充质干细胞（mesenchymal stem cells, MSCs）的基因表达谱展开了对比分析。研究团队采用多光束激光干涉法在聚酰亚胺材料上制备了该图案结构。此类结构可影响人间充质干细胞的细胞尺寸与定向排布。进一步研究发现，周期为650 nm的纳米图案可促进MSCs向成骨细胞系与脂肪细胞系分化。然而，在未添加分化培养基的条件下，经Affymetrix基因芯片（Affymetrix microarray）技术开展全基因组基因表达谱分析后证实，该表面结构既未诱导细胞分化，也未引发谱系特异性基因表达变化。本研究结果表明，周期为650 nm的沟槽/脊结构可增强MSCs向脂肪细胞系和/或成骨细胞系分化的潜能，但并未直接调控谱系特异性基因表达的改变。
整体实验设计：共9个样本与GeneChip人类基因2.0 ST芯片（Affymetrix）进行杂交。与本手稿中的标注相对应，供体1对应AT57，供体2对应AT58，供体3对应AT61。