Rattus norvegicus Long Evans transcriptome project

Left and right rat (postnatal 5 weeks) dorsal hippocampal CA3 region. 3 sets of paired (left vs. right CA3) samples are made. The number of animals used in each sample is 36, 57, and 34. Total RNA amount of 3.0 ug per sample was used. Small RNAs were purified using a miRNeasy (Qiagen) kit. Small RNA cDNA library was constructed according to Kawano et al (2010) Biotechniques, 49: 751-755. The number of PCR cycles for small RNA cDNA library construction is 12. The concentration of each purified small RNA cDNA library was determined using Agilent 2100 BioAnalyzer with a DNA1000 Labchip (Agilent) and adjusted to 6 pM for sequencing. The small RNA cDNA libraries were sequenced using Genome Analizer IIx.

大鼠出生后5周的左侧与右侧背侧海马CA3区。共制备3组配对样本（左侧CA3与右侧CA3），每组样本对应的实验动物数量分别为36、57和34。每个样本取用3.0 μg总RNA。采用miRNeasy（Qiagen）试剂盒纯化小RNA。参照Kawano等人2010年发表于《Biotechniques》第49卷第751-755页的实验方法构建小RNA cDNA文库。小RNA cDNA文库构建过程中的PCR循环数为12。采用Agilent 2100生物分析仪搭配DNA1000实验芯片（Agilent）对各纯化后的小RNA cDNA文库进行浓度测定，并将文库浓度调整至6 pM以用于测序。采用Genome Analizer IIx对小RNA cDNA文库进行测序。