Research data for paper 'Time dependent differential regulation of a novel long non-coding natural antisense RNA during long-term memory formation'.

Behavioural data used in paper published in Scientific Reports

Dataset for Fig. 4a: The results of the behavioural test of long-term memory (LTM) formation at 24 h after single-trial food reward classical conditioning. The data shown are difference scores calculated by subtracting the number of spontaneous feeding rasps counted in a two-minute period before the application of the conditioned stimulus from those counted in the two-minute period after the conditioned stimulus was applied.

Abstract

Long natural antisense transcripts (NATs) have been demonstrated in significant numbers in a variety of eukaryotic organisms. They are particularly prevalent in the nervous system suggesting their importance in neural functions. However, the precise physiological roles of the overwhelming majority of long NATs remain unclear. Here we report on the characterization of a novel molluscan nitric oxide synthase (NOS)-related long non-coding NAT (Lym-NOS1AS). This NAT is spliced and polyadenylated and is transcribed from the non-template strand of the Lym-NOS1 gene. We demonstrate that the Lym-NOS1AS is co-expressed with the sense Lym-NOS1 mRNA in a key neuron of memory network. Also, we report that the Lym-NOS1AS is spatially and temporally regulated by one-trial conditioning leading to long-term memory (LTM) formation. Specifically, in the cerebral, but not in the buccal ganglia, the temporal pattern of changes in Lym-NOS1AS expression after training correlates well with the alternation of memory lapse and non-lapse periods. Our data suggest that the Lym-NOS1AS plays a role in the consolidation of nitric oxide-dependent LTM.

发表于《Scientific Reports》的论文中使用的行为学数据集。

图4a对应数据集：单次食物奖赏经典条件反射后24小时的长时记忆（long-term memory, LTM）形成行为学测试结果。本数据集展示的为差值得分，计算方式为：用施加条件刺激后2分钟内记录到的自发性摄食刮食动作次数，减去施加条件刺激前2分钟内记录到的对应次数。

摘要
长链天然反义转录本（long natural antisense transcripts, NATs）已在多种真核生物中被大量鉴定，且在神经系统中尤为富集，提示其在神经功能中发挥重要作用。然而，绝大多数长链天然反义转录本的确切生理功能仍未明确。本研究对一种新型软体动物源一氧化氮合酶（nitric oxide synthase, NOS）相关长链非编码天然反义转录本（Lym-NOS1AS）进行了表征。该天然反义转录本经过剪接并带有多聚腺苷酸尾，从Lym-NOS1基因的非模板链转录而来。研究证实，Lym-NOS1AS与正义链Lym-NOS1 mRNA在记忆网络的关键神经元中共表达。此外，本研究发现Lym-NOS1AS可被诱导长时记忆形成的单次条件化训练进行时空调控。具体而言，在脑神经节（而非口神经节）中，训练后Lym-NOS1AS表达的时间变化模式与记忆消退期和非消退期的交替高度吻合。本研究数据表明，Lym-NOS1AS参与了一氧化氮依赖型长时记忆的巩固过程。