Comparison between HPV+ / p16+ versus the HPV-/p16- from HNSCC patients. Comparison between HPV+ / p16+ versus the HPV-/p16- from HNSCC patients

Head and neck squamous cell carcinomas are heterogeneous neoplasms that show clinical and biological differences in association with the human papillomavirus (HPV). To provide adequate therapeutic strategies, biological and clinical characterization is essential to stratify patients based on prognostic and predictive factors. Reports on HNSCC are scarce in Mexico, thus in the present study, we analyze 414 cases of HNSCC, including those of the oropharynx (OPSCC), larynx (LASCC), and oral cavity (OCSCC). We determined the presence of HPV and p16 expression. Global expression profiles were analyzed with Affymetrix HTA 2.0 microarray in 25 selected cases HPV+/p16+ versus HPV-/p16-. In our study we analyze 25 samples derived from HNSCC patients. 18 samples were HPV-/p16- and 7 HPV+/p16+. We compared the HPV-/p16- versus the HPV+/p16+ and identified differentially expressed RNAs with a fold change greater than 1.5 or less than -1.5. These data were used to obtain 98 genes that are differentially expressed in absence of HPV. The present study offers information regarding clinical and molecular characteristics of HNSCC, both associated and unassociated with HPV, in Mexican patients. Overall design: 25 total samples were analyzed. The samples were collected from Unidad Médica de Alta Especialidad de Oncología del Centro Médico Nacional Siglo XXI, of the Instituto Mexicano del Seguro Social (Mexican Social Security Institute). We compared HPV+/p16+ and HPV-/p16- of patients with HNSCC . A fold change greater than 1.5 or less than -1.5 and a p-value ≤ 0.05 were used to compare gene expression.

头颈部鳞状细胞癌（Head and neck squamous cell carcinomas, HNSCC）是一类异质性肿瘤，其临床与生物学特征与人乳头瘤病毒（human papillomavirus, HPV）感染密切相关。为制定合理的治疗策略，基于预后与预测因子对患者进行分层至关重要。鉴于墨西哥地区关于头颈部鳞状细胞癌的研究报道较为匮乏，本研究纳入414例头颈部鳞状细胞癌病例，涵盖口咽部鳞状细胞癌（oropharyngeal squamous cell carcinoma, OPSCC）、喉鳞状细胞癌（laryngeal squamous cell carcinoma, LASCC）与口腔鳞状细胞癌（oral cavity squamous cell carcinoma, OCSCC）。我们首先检测了上述病例的HPV感染与p16蛋白表达情况，随后从中选取25例患者样本开展全基因表达谱分析，其中18例为HPV阴性/p16阴性，7例为HPV阳性/p16阳性。本研究采用Affymetrix HTA 2.0基因芯片对该25例样本进行全基因表达谱检测，并对比HPV阴性/p16阴性与HPV阳性/p16阳性样本的基因表达差异，筛选出折叠变化大于1.5或小于-1.5的差异表达RNA，最终获得98个在HPV阴性状态下差异表达的基因。本研究为墨西哥头颈部鳞状细胞癌患者中与HPV相关及不相关的肿瘤的临床与分子特征提供了详实数据。实验设计概述：本研究共分析25例样本，所有样本均采集自墨西哥社会保障研究所（Instituto Mexicano del Seguro Social, IMSS）国家医学中心Siglo XXI的肿瘤学高等专科医疗单元。我们以折叠变化大于1.5或小于-1.5且p值≤0.05作为基因表达差异的筛选标准，对头颈部鳞状细胞癌患者的HPV阳性/p16阳性与HPV阴性/p16阴性样本进行比较分析。