MPG Ranch 16S amplicon data

16S amplicon data from soil study at MPG Ranch. Dates in file names denote sequencing runs at Argonne National Lab. Files beginning with R1 are forward read files (raw fastq files) and files beginning with I1 are index sequences. Concatenate the R1 and I1 files with the same date (in alphabetical order) prior to demultiplexing. Mapping files with barcodes are also included (text files). Dates in mapping file correspond to dates in sequence files. Demultiplex each sequencing run (date) seperately. Full metadata mapping file for all samples is also included. Open reference OTU table (97% identity, singletons removed), taxonomy metadata file, OTU representative sequence fasta file, and phylogenetic tree file also included.

本数据集为MPG牧场土壤研究的16S扩增子测序数据。文件名中附带的日期用于标识阿尔贡国家实验室（Argonne National Lab）的各次测序批次。以R1开头的文件为正向测序读段原始FASTQ文件，以I1开头的文件为索引序列文件。在进行多重测序拆分（demultiplexing）前，需将同一日期的R1与I1文件按字母顺序拼接。本次数据集还附带包含条形码的映射文件（文本格式），映射文件中的日期与测序文件中的日期一一对应。需对每个按日期划分的测序批次单独进行多重测序拆分操作。此外还提供了覆盖所有样本的完整元数据映射文件。同时包含已移除单例序列的97%相似度开放参考型操作分类单元（Operational Taxonomic Unit）表、分类元数据文件、OTU代表序列FASTA格式文件以及系统发育树文件。