Reprogram-Seq: A platform for single-cell combinatorial reprogramming [I]

Reprogram-Seq leverages organ-specific cell atlas data with single-cell perturbation and computational analysis to predict, evaluate, and optimize TF combinations that reprogram a cell type of interest. Overall design: Focusing on the cardiac system, we performed Reprogram-Seq on P0 mouse heart cells to generate a reference transcriptomic map. Based on the reference map, we selected TF candidates and tests 1000s of TF cocktails for direct lineage conversion by scRNA-Seq.

重编程测序（Reprogram-Seq）依托器官特异性细胞图谱数据集、单细胞扰动实验与计算分析手段，实现对目标细胞类型重编程所需转录因子（Transcription Factor, TF）组合的预测、评估与优化。整体实验设计：本研究以心脏系统为研究对象，对出生后第0天（P0）小鼠心脏细胞开展重编程测序（Reprogram-Seq）以构建参考转录组图谱。基于该参考图谱，我们筛选得到候选转录因子，并通过单细胞RNA测序（single-cell RNA sequencing, scRNA-Seq）对数千种转录因子组合方案开展直接谱系转换实验验证。