Effect of neutrophil extracellular vesicles treatment on the K/BxN mouse model of arthritis

Arthritis was induced in C57BL/6 mice by injection of arthritogenic serum. This study aims to evaluate the transcriptomic profile of the K/BxN model of arthritis in knee joints, as well as he anti-inflammatory/tissue pro-repair effect of the intra-articular administration of neutrophil extracellular vesicles (EVs). Overall design: Four study groups were included: Disease (arthritic, n=4); Naive (healthy, n=4); Treated (arthritic, treated with EVs, n=8); Control (arthritic, treated with vehicle, n=8). Arthritis was induced with two injections of K/BxN arthritigenic serum on days 0 and 2. Treatments were applied on day 3. Mice were sacrificed on day 10.

本研究通过注射致关节炎血清，于C57BL/6小鼠体内诱导关节炎模型。本研究旨在评估膝关节K/BxN关节炎模型的转录组谱，以及关节腔内给予中性粒细胞胞外囊泡（neutrophil extracellular vesicles, EVs）所产生的抗炎/组织促修复效应。实验设计概况：共设置4组受试对象：疾病组（关节炎造模小鼠，n=4）；未造模组（健康小鼠，n=4）；治疗组（关节炎造模小鼠，经EVs治疗，n=8）；载体对照组（关节炎造模小鼠，经载体处理，n=8）。造模采用在第0天和第2天两次注射K/BxN致关节炎血清的方式，治疗于第3天实施，所有小鼠于第10天处死。