Transcriptome profile of MafB-cKO of podocyte. Mus musculus

The transcription factor MafB is essential for differentiation and foot process formation of podocytes. In order to identify the downstream targets of MafB, we analyzed the Mafb-deficient podocyte by RNA-seq. We found slit diaphragm-related protein (Nphs1, Magi2), Rho GTPase-activating protein (Arhgap24, Iqgap2) and podocyte-specific transcription factor (Tcf21) were significantly reduced in MafB cKO glomeruli. This indicates that one of these factors might be directly regulated by MafB to maintain its function in podocyte maintenance. Overall design: Examination of 6 samples (3 Control and 3 Mafb-cKO podocyte ). Each sample contains RNA from 1 mouse. Paired-end RNA sequencing was performed with NextSeq500 sequencer (Illumina).

转录因子MafB对于足细胞的分化与足突形成至关重要。为鉴定MafB的下游靶基因，我们通过RNA测序（RNA-seq）对Mafb缺陷型足细胞开展了转录组分析。结果显示，在Mafb条件性敲除（cKO）肾小球中，裂孔隔膜相关蛋白（Nphs1、Magi2）、Rho GTP酶激活蛋白（Arhgap24、Iqgap2）以及足细胞特异性转录因子（Tcf21）的表达水平显著下调。该结果提示，上述部分因子可能直接受MafB调控，从而维持足细胞的正常功能。总体实验设计：共检测6个样本（3个对照组与3个Mafb条件性敲除组足细胞样本），每个样本包含1只小鼠的RNA。使用Illumina NextSeq500测序仪完成双端RNA测序。