CX43 heterozygous, Cx43 null and Cx32 null vs wildtype neonatal mouse brain

Cy3-labeled cDNA from brains of neonatal C57BL Cx43 null, Cx43 heterozygous and Cx32 null mice were compared among themselves and to Cy3-labeled cDNA from brains of neonatal C57BL wildtype mice through Cy5-labeled sample reference prepared at once for the entire experiment from aorta, brain, heart, kidney, liver, lung, ovary/testicles, spleen, and stomach - equal amounts from adult male and female C57BL mice.

本实验以一次性制备的Cy5标记混合样本作为全程参照：该参照样本取自成年雌雄C57BL小鼠的主动脉、脑、心脏、肾脏、肝脏、肺、卵巢/睾丸、脾脏及胃，各组织取材量均等且雌雄配比一致。将新生C57BL背景下Cx43纯合缺失、Cx43杂合子及Cx32纯合缺失小鼠的脑组织Cy3标记互补DNA（cDNA）彼此之间，以及与新生C57BL野生型小鼠的脑组织Cy3标记cDNA进行对比分析。