Integrative analysis of differentially expressed genes and miRNAs predicts complex T3-mediated protective circuits in a rat model of cardiac ischemia reperfusion. Integrative analysis of differentially expressed genes and miRNAs predicts complex T3-mediated protective circuits in a rat model of cardiac ischemia reperfusion

mRNA and miRNA expression profiling shows that the T3 supplementation reverted the expression of 87 genes and 11 miRNAs that were dysregulated in the untreated group Overall design: A rat model of myocardial IR, with or without an early short-term T3-replacement, was used to predict putative T3-dependent miRNA-gene interactions targeted to mitochondria quality control and wound healing repair. One Sham, two IR, and two IRT3 rats were collected for mirna profiling.

信使RNA（messenger RNA, mRNA）与微小RNA（microRNA, miRNA）表达谱分析结果显示，三碘甲状腺原氨酸（triiodothyronine, T3）补充处理可使未处理组中失调的87个基因与11个miRNA的表达恢复至正常水平。实验设计概况：本研究采用伴或不伴早期短期T3替代治疗的大鼠心肌缺血再灌注（myocardial ischemia-reperfusion, IR）模型，用于预测靶向线粒体质量控制与创伤愈合修复的、推测的T3依赖型miRNA-基因互作调控关系。本研究共收集1只假手术组大鼠、2只缺血再灌注组大鼠以及2只缺血再灌注联合T3处理组大鼠，用于miRNA表达谱分析。