The Molecular Dissection of the Oncogenic Role of ETS1 in the Mesenchymal Subtypes of Head and Neck Squamous Cell Carcinoma [ChIP-seq]

Mapping the genome-wide binding profile of ETS1 in a Mesenchymal HNSCC Cell Line using ChIP-Seq techniques Overall design: The global binding loci of ETS1 in SCC25 cells, were mapped using ChIP-Sequencing analysis. We performed three replicate experiments of high quality, using two different antibodies, C20 (Santa Cruz Biotechnology) and 501A (Bethyl Laboratories), and consensus binding events were determined using IDR analysis. The ETS1 binding profiles in SCC25 were intersected with the global ETS1 Knockdown RNA-Seq analysis in order to identify the direct transcriptional targets of ETS1 in a Mesenchymal model of HNSCC.

利用染色质免疫沉淀测序（ChIP-Seq）技术绘制间质型头颈鳞状细胞癌（HNSCC）细胞系中ETS1的全基因组结合谱。实验设计：本研究通过染色质免疫沉淀测序（ChIP-Seq）分析，定位SCC25细胞内ETS1的全基因组结合位点。我们使用两种不同的抗体——C20（Santa Cruz Biotechnology）与501A（Bethyl Laboratories）——开展了3次高质量重复实验，并通过不可重复发现率（IDR）分析确定了共识结合事件。为鉴定间质型头颈鳞状细胞癌（HNSCC）模型中ETS1的直接转录靶标，我们将SCC25细胞的ETS1结合谱与全基因组ETS1敲低RNA测序（RNA-Seq）分析结果进行了交集分析。