SOX2 regulates foregut squamous epithelial homeostasis and is lost during Barrett’s esophagus development [SOX2mousetissue]

Differentially expressed genes in SOX2 KO vs control forestomachs In this dataset, we include expression data from 4 SOX2 KO forestomachs and 3 control forestomaches In this dataset, we include expression data from 4 SOX2 KO forestomachs and 3 control forestomachs. To induce gene deletion but not gastric injury, “low-dose” tamoxifen (1 mg/20 g body weight) was injected intraperitoneally for 7 consecutive days. Mouse forestomach tissues from four Krt5CreER/+; Sox2∆/∆; ROSA26tdTomato/+ mice and three wildtype control mice were harvested and flushed with PBS. Corpus and antrum were removed. The forestomach tissues were mechanically dissociated using a tissue Homogenizer 850. RNA was isolated using RNeasy Mini Kit, following the manufacturer’s protocol. For microarray, samples were processed and hybridized to Affymetrix Mouse Gene 2.0 ST by the Washington University Genome Technology Access Core (GTAC). Mouse forestomach tissue GeneChips were analyzed with Partek Flow Genomic Analysis Software using default settings for array QC, data normalization, and to generate differential expression analyses including hierarchical clustering and volcano plots.

SOX2基因敲除（SOX2 KO）与对照小鼠前胃组织的差异表达基因
本数据集包含4例SOX2基因敲除小鼠前胃组织与3例对照小鼠前胃组织的基因表达谱数据。为诱导基因敲除而非胃组织损伤，我们采用低剂量他莫昔芬（tamoxifen，1 mg/20 g体重）连续7天腹腔注射造模。我们收集了4只Krt5CreER/+; Sox2∆/∆; ROSA26tdTomato/+基因工程小鼠及3只野生型对照小鼠的前胃组织，经磷酸盐缓冲液（PBS）冲洗后去除胃体与胃窦部分。使用组织匀浆器850（Tissue Homogenizer 850）对前胃组织进行机械解离，随后按照制造商说明书采用RNeasy Mini Kit提取总RNA。芯片样本的处理及与Affymetrix Mouse Gene 2.0 ST基因芯片的杂交实验均由华盛顿大学基因组技术获取核心（Washington University Genome Technology Access Core，GTAC）完成。采用Partek Flow Genomic Analysis Software，以默认参数对小鼠前胃组织基因芯片进行阵列质控、数据标准化及差异表达分析，分析内容包含层级聚类与火山图绘制。