Additional file 14 of Changes in ADAR RNA editing patterns in CMV and ZIKV congenital infections

Additional file 14: Supplementary File 12. Number of (and percent of unmapped) reads that were mapped to the respective viral genomes. Briefly, unmapped reads were collected from the BAM files and mapped to the viral genomes of MCMV and ZIKV using STAR. The following reference genomes were used: NC_075725 (MCMV), KX520666 (ZIKV1), KU866423 (ZIKV2), and KX197192 and MH158236* for human ZIKV dataset, respectively. As expected, the viral reads were found primarily in the infected samples, although their numbers represented only relatively small portions of all sequenced reads. A handful of viral reads were also detected in some uninfected samples, consistent with previously reported results (Lima et al. 2019, Fig. 4C) and the possibility of artifacts of mapping and/or negligible contamination. * MH158236 is a complete genome of JN860885 (FSS13025) ZIKV isolate.

附加文件14：补充文件12。比对至对应病毒基因组的读段数量（及未比对读段占比）。简言之，从BAM文件（Binary Alignment Map）中提取未比对读段，使用STAR比对软件将其比对至小鼠巨细胞病毒（MCMV）与寨卡病毒（ZIKV）的基因组。本次分析所用参考基因组如下：NC_075725（对应小鼠巨细胞病毒MCMV）、KX520666（对应ZIKV1）、KU866423（对应ZIKV2），以及用于人类ZIKV数据集的KX197192与MH158236*，各基因组均按标注对应关系使用。正如预期，病毒读段主要存在于感染样本中，但其仅占所有测序读段的相对较小比例。部分未感染样本中也检测到少量病毒读段，这与此前发表的研究结果（Lima等，2019，图4C）一致，同时提示存在比对伪影或可忽略污染的可能性。* MH158236为JN860885（FSS13025）ZIKV分离株的完整基因组。