RNA-seq of ESCC Cells Overexpressed TFAP2ß or its LLPS-deficient Mutants

This dataset contains raw RNA-seq data from esophageal squamous cell carcinoma (ESCC) cells overexpressing GFP-TFAP2ß, its LLPS-deficient mutants (?IDR3 and Mut_IDR3) or GFP control. The experiment aims to explore the downstream genes of TFAP2ß and its phase separation-deficient mutants to uncover the role of TFAP2ß's LLPS activity in regulating gene expression in ESCC. Data were collected from the genomic binding of TFAP2ß and its LLPS-deficient mutants, providing insights into the regulatory networks controlled by TFAP2ß in cancer progression. Overall design: RNA-seq of TFAP2ß, its LLPS-deficient mutants (?IDR3 and Mut_IDR3) or GFP control in ESCC cells KYSE150 to understand the downstream genes and regulatory activity associated with TFAP2ß's phase separation mechanism.

本数据集包含过表达绿色荧光蛋白（Green Fluorescent Protein, GFP）标记的转录因子AP-2β（TFAP2β）、其液-液相分离（liquid-liquid phase separation, LLPS）缺陷型突变体（ΔIDR3与Mut_IDR3）以及GFP空载体对照的食管鳞状细胞癌（esophageal squamous cell carcinoma, ESCC）细胞的原始RNA测序（RNA-seq）数据。本实验旨在探究TFAP2β及其相分离缺陷型突变体的下游靶基因，以阐明TFAP2β的LLPS活性在食管鳞状细胞癌基因表达调控中发挥的作用。本数据集采集了TFAP2β及其LLPS缺陷型突变体的基因组结合位点相关数据，为揭示TFAP2β在癌症进展中调控的基因网络提供了研究思路。实验整体设计：在食管鳞状细胞癌KYSE150细胞中，对过表达TFAP2β、其LLPS缺陷型突变体（ΔIDR3与Mut_IDR3）以及GFP空载体对照的样本开展RNA-seq测序，以解析与TFAP2β相分离机制相关的下游靶基因及调控活性。