Proteome Profiling in the Rat Dorsal Striatum Synaptoneurosome Following Methamphetamine Exposure

We performed discovery-based proteomic profiling of synaptoneurosomes (SNs), subcellular fractions of brain tissue containing both pre- and post-synaptic components, isolated from the dorsal striatum of rats following methamphetamine (Meth) administration to investigate how methamphetamine exposure alters the synaptic proteome. Using tandem mass tag (TMT)-based multiplexed quantification with high-resolution mass spectrometry (HRMS), we identified 6094 proteins and quantified 4583 in 3 out 5 biological replicates. Comparative analysis between Meth-treated and control rats revealed differential regulation for 147 proteins. These proteins were enriched in synaptic, mitochondrial, and proteasomal pathways.

我们对突触神经小体（synaptoneurosomes, SNs）开展了发现驱动的蛋白质组学分析；该亚细胞组分源自脑组织，包含突触前与突触后所有成分，分离自经甲基苯丙胺（methamphetamine, Meth）处理的大鼠背侧纹状体，本研究旨在探究甲基苯丙胺暴露如何改变突触蛋白质组。本研究采用基于串联质量标签（tandem mass tag, TMT）的多重定量技术结合高分辨质谱（high-resolution mass spectrometry, HRMS），在5次生物学重复中的3次重复内，共鉴定到6094种蛋白质，并完成了其中4583种的定量分析。对甲基苯丙胺处理组与对照组大鼠的比较分析显示，共有147种蛋白质呈现差异调控。上述差异调控蛋白质显著富集于突触、线粒体及蛋白酶体通路。