Fast and Ecological Liquid-Liquid Separation Method for Preparing Quinones Enriched Extract from Nigella sativa Oil

A fast, inexpensive, and ecological method based on liquid-liquid extraction principle has been developed for preparation of extract with high content of Nigella sativa (N. sativa) quinones. The gas chromatography-mass spectrometry (GC-MS) analysis showed that oil extraction using 70% ethanol for 6 h yielded extract containing 50% of thymoquinone (TQ) and 13% of thymohydroquinone (THQ).本研究开发了一种基于液液萃取（liquid-liquid extraction）原理的快速、低成本且绿色环保的方法，用于制备高含量黑种草（Nigella sativa, N. sativa）醌类提取物。经气相色谱-质谱联用法（gas chromatography-mass spectrometry, GC-MS）分析证实，采用70%乙醇萃取6小时所得的提取物中，百里醌（thymoquinone, TQ）占比达50%，百里氢醌（thymohydroquinone, THQ）占比达13%。