Data from: Direct and trans-generational effects of male and female gut microbiota in Drosophila melanogaster

There is increasing evidence of the far-reaching effects of gut bacteria on physiological and behavioural traits, yet the fitness-related consequences of changes in the gut bacteria composition of sexually interacting individuals remain unknown. To address this question, we manipulated the gut microbiota of fruit flies, Drosophila melanogaster, by monoinfecting flies with either Acetobacter pomorum (AP) or Lactobacillus plantarum (LP). Re-inoculated individuals were paired in all treatment combinations. LP-infected males had longer mating duration and induced higher short-term offspring production in females compared with AP-infected males. Furthermore, females of either re-inoculation state mated with AP-infected males were more likely to have zero offspring after mating, suggesting a negative effect of AP on male fertility. Finally, we found that the effects of male and female gut bacteria interacted to modulate their daughters', but not sons' body mass, revealing a new trans-generational effect of parental gut microbiota. In conclusion, this study shows direct and trans-generational effects of the gut microbiota on mating and reproduction.

Usage Notes
Morimotoetal2016-gutmicrobiota-BehaviouraldataBehavioural data of the manipulation of the gut bacteria on mating and reproductive parameters. Tab 2 contains the data for the offspring body mass.Morimotoetal2016-gutmicrobiota.xls

越来越多的证据表明，肠道菌群对生理和行为特征具有深远的影响，然而，性互动个体肠道菌群组成变化对适应性后果仍知之甚少。为解决这一问题，我们通过单菌感染果蝇Drosophila melanogaster的肠道菌群，分别以醋酸菌（Acetobacter pomorum，AP）或植物乳杆菌（Lactobacillus plantarum，LP）感染果蝇。在所有处理组合中，再接种个体均被配对。与AP感染的雄性相比，LP感染的雄性在交配时间上更长，并能诱导雌性产生更高的短期后代产量。此外，与AP感染的雄性交配的雌性，无论再接种状态如何，在交配后更可能没有后代，这表明AP对雄性生育力的负面影响。最后，我们发现，男性和女性的肠道菌群相互作用，调节她们女儿而非儿子的体重，揭示了父代肠道菌群对后代的新跨代效应。总之，本研究揭示了肠道菌群对交配和繁殖的直接及跨代效应。

使用说明

Morimotoetal2016-gutmicrobiota-Behaviouraldata
行为数据：肠道菌群对交配和繁殖参数的操纵数据。表2包含后代体重的数据。
Morimotoetal2016-gutmicrobiota.xls