Cryptic developmental events determine medulloblastoma radiosensitivity and cellular heterogeneity without altering transcriptomic profile. Cryptic developmental events determine medulloblastoma radiosensitivity and cellular heterogeneity without altering transcriptomic profile

Using scRNA-seq, we show in a murine medulloblastoma model that tumors initiated earlier in development differ from later initiated tumors in their cellular composition and response to radiation therapy. Overall design: Brains were cut in half sagittally and drop-fixed in 4% paraformaldehyde for IHC, and half cerebellum or tumor collected for Drop-Seq.

本研究通过单细胞RNA测序（single-cell RNA sequencing, scRNA-seq），在小鼠髓母细胞瘤模型中证实：发育早期始发的肿瘤与发育晚期始发的肿瘤在细胞组成及放射治疗应答反应方面存在显著差异。实验设计概述：将大脑沿矢状面切为两半，其中一半采用4%多聚甲醛滴液固定以用于免疫组化（immunohistochemistry, IHC）检测，另一半小脑或肿瘤组织则被收集用于Drop-Seq测序。