Data_Sheet_1_Tumor-Associated Microbiota in Esophageal Squamous Cell Carcinoma.PDF

Important evidence indicates the microbiota plays a key role in esophageal squamous cell carcinoma (ESCC). The esophageal microbiota was prospectively investigated in 18 patients with ESCC and 11 patients with physiological normal (PN) esophagus by 16S rRNA gene profiling, using next-generation sequencing. The microbiota composition in tumor tissues of ESCC patients were significantly different from that of patients with PN tissues. The ESCC microbiota was characterized by reduced microbial diversity, by decreased abundance of Bacteroidetes, Fusobacteria, and Spirochaetes. Employing these taxa into a microbial dysbiosis index demonstrated that dysbiosis microbiota had good capacity to discriminate between ESCC and PN esophagus. Functional analysis characterized that ESCC microbiota had altered nitrate reductase and nitrite reductase functions compared with PN group. These results suggest that specific microbes and the microbiota may drive or mitigate ESCC carcinogenesis, and this study will facilitate assigning causal roles in ESCC development to certain microbes and microbiota.

重要证据表明，微生物群在食管鳞状细胞癌（esophageal squamous cell carcinoma，ESCC）中发挥关键作用。本研究采用下一代测序（next-generation sequencing）技术，通过16S核糖体RNA（16S ribosomal RNA，16S rRNA）基因谱分析，对18例ESCC患者与11例食管生理正常（physiological normal，PN）受试者的食管微生物群进行了前瞻性研究。ESCC患者肿瘤组织的微生物群组成与PN组受试者食管组织的微生物群组成存在显著差异。ESCC相关微生物群以微生物多样性降低、拟杆菌门（Bacteroidetes）、梭杆菌门（Fusobacteria）及螺旋体门（Spirochaetes）丰度下降为特征。将上述分类群纳入微生物失调指数后，结果显示失调的微生物群可较好地区分ESCC患者与PN组受试者的食管组织。功能分析显示，与PN组相比，ESCC相关微生物群的硝酸盐还原酶（nitrate reductase）与亚硝酸盐还原酶（nitrite reductase）功能发生了改变。上述结果表明，特定微生物与微生物群可能促进或抑制ESCC的发生发展，本研究将有助于明确特定微生物及微生物群在ESCC发生发展中的因果作用。